| James Cagney | George M. Cohan |
| Joan Leslie | Mary |
| Walter Huston | Jerry Cohan |
| Richard Whorf | Sam Harris |
| Irene Manning | Fay Templeton |
| George Tobias | Dietz |
| Rosemary DeCamp | Nellie Cohan |
| Jeanne Cagney | Josie Cohan |
| Frances Langford | Singer |
| George Barbier | Erlanger |
| S.Z. Sakall | Schwab |
| Walter Catlett | Theatre Manager |
| Douglas Croft | George M. Cohan - As a Boy of 13 |
| Eddie Foy Jr. | Eddie Foy |
| Minor Watson | Albee |
| Chester Clute | Goff |
| Odette Myrtil | Madame Bartholdi |
| Patsy Parsons | Josie Cohan - As a Girl of 12 (as Patsy Lee Parsons) |
| Jack Young | The President (as Capt. Jack Young) |
| Eddie Acuff | Reporter (uncredited) |
| Murray Alper | Wiseguy (uncredited) |
| Ernest Anderson | George M. Cohan's Valet (uncredited) |
| Vivian Austin | Pianist (uncredited) |
| Leah Baird | Housekeeper (uncredited) |
| Leon Belasco | Magician (uncredited) |
| Brooks Benedict | Dressing Room Guest (uncredited) |
| Henry Blair | George M. Cohan at 7 (uncredited) |
| Walter Brooke | Reporter (uncredited) |
| Leslie Brooks | Chorus Girl - 'Little Johnny Jones' Number (uncredited) |
| Georgia Caine | Boarder (uncredited) |
| Georgia Carroll | Betsy Ross (uncredited) |
| Glen Cavender | Colony Opera House Stagehand (uncredited) |
| Dick Chandlee | Teenager (uncredited) |
| Spencer Charters | Colony Opera House Stage Manager (uncredited) |
| Wallis Clark | Theodore 'Teddy' Roosevelt (uncredited) |
| Alan Copeland | Choirboy (uncredited) |
| Ann Corcoran | Soubrette (uncredited) |
| Mary Currier | Woman Entering Cohan's Dressing Room (uncredited) |
| William B. Davidson | New York Stage Manager (uncredited) |
| Frank Dee | Man Entering Cohan's Dressing Room (uncredited) |
| Ann Doran | Receptionist (uncredited) |
| Charles Drake | Actor (uncredited) |
| Tom Dugan | Actor - Railroad Station (uncredited) |
| Ann Edmonds | Soubrette (uncredited) |
| Bill Edwards | Reporter (uncredited) |
| Frank Faylen | Sergeant on Parade - Last Scene (uncredited) |
| Pat Flaherty | Sgt. Lewis - White House Guard (uncredited) |
| Robert Flatley | Dancer (uncredited) |
| James Flavin | Union Army Veteran #1 on Caisson (uncredited) |
| William Forrest | Critic #1 (uncredited) |
| William Gillespie | Baritone Solo - Grand Old Flag Number (uncredited) |
| Art Gilmore | Franklin D. Roosevelt (voice) (uncredited) |
| Eddie Graham | Actor (uncredited) |
| Joe Gray | (uncredited) |
| Creighton Hale | Telegraph Operator (uncredited) |
| John Hamilton | Recruiting Major (uncredited) |
| Harry Hayden | Dr. Llewellyn (uncredited) |
| Al Herman | Actor (uncredited) |
| Herbert Heywood | Colony Opera House Doorman (uncredited) |
| Stuart Holmes | Backstage Actor - 'Peck's Bad Boy' (uncredited) |
| William Hopper | Reporter (uncredited) |
| Joyce Horne | Teenager (uncredited) |
| Jean Inness | Reporter (uncredited) |
| Charles Irwin | Horse Race Announcer - 'Little Johnny Jones' Number (uncredited) |
| Thomas E. Jackson | Stage Manager - 'Peck's Bad Boy' (uncredited) |
| Marijo James | Sister Act (uncredited) |
| Eddie Kane | Wilson - 'Little Johnny Jones' Number (uncredited) |
| Edward Keane | Critic #2 (uncredited) |
| Dorothy Kelly | Sister Act (uncredited) |
| Fred Kelsey | Irish Cop in 'Peck's Bad Boy' (uncredited) |
| Phyllis Kennedy | Fanny (uncredited) |
| Joe Levine | Newsboy (uncredited) |
| Vera Lewis | Actress (uncredited) |
| Al Lloyd | Actor (uncredited) |
| Audrey Long | Dietz and Goff's Receptionst (uncredited) |
| Jerrie Lynne | Singer (uncredited) |
| Hank Mann | Peck's Bad Boy Stagehand (uncredited) |
| Jo Ann Marlowe | Josie Cohan - Age 6 (uncredited) |
| Louis Mason | Boarder (uncredited) |
| Frank Mayo | Hotel Clerk #2 (uncredited) |
| Lon McCallister | Call Boy (uncredited) |
| Edward McWade | New York Stage Doorman (uncredited) |
| George Meeker | Hotel Clerk #1 (uncredited) |
| June Millarde | Young Girl (uncredited) |
| John 'Skins' Miller | Horse Race Official (uncredited) |
| Frank Mills | Pedestrian Seeking Newspaper (uncredited) |
| Bert Moorhouse | Maurice Ruppe - Music Publisher (uncredited) |
| Dolores Moran | Girl (uncredited) |
| Charles Morton | Friendly Man at Restaurant Window on New Year's Eve (uncredited) |
| Jack Mower | Backstage Actor - 'Peck's Bad Boy' (uncredited) |
| Lee Murray | Jockey (uncredited) |
| George Ovey | Streetcleaner (uncredited) |
| Garry Owen | Army Clerk (uncredited) |
| Paul Panzer | Robinsons Theater Stagehand (uncredited) |
| Francis Pierlot | Dr. Anderson (uncredited) |
| Joyce Reynolds | Teenager (uncredited) |
| Ruth Robinson | Nurse (uncredited) |
| Clinton Rosemond | White House Butler (uncredited) |
| Thomas W. Ross | Doctor (uncredited) |
| Jackie Salling | Newsboy (uncredited) |
| Fred Santley | Judge in Musical Number (uncredited) |
| Syd Saylor | Star Boarder (uncredited) |
| Harry Seymour | O'Rourke's Varieties Stagehand (uncredited) |
| John Sheehan | Boarder (uncredited) |
| Napoleon Simpson | Porter (uncredited) |
| Charles Smith | Teenager (uncredited) |
| Ernie Stanton | Waiter (uncredited) |
| Juanita Stark | Soubrette (uncredited) |
| Elliott Sullivan | Army Recruiter Examiner (uncredited) |
| Frank Sully | Army Recruiter (uncredited) |
| Jim Toney | Actor (uncredited) |
| Sailor Vincent | Schults - Grocer in 'Peck's Bad Boy' (uncredited) |
| Dick Wessel | Union Army Veteran #2 on Caisson (uncredited) |
| Leo White | Backstage Actor - 'Peck's Bad Boy' (uncredited) |
| Poppy Wilde | Chorus Girl - 'Little Johnny Jones' Number (uncredited) |
| Dave Willock | Stage Manager, 'Peck's Bad Boy' (uncredited) |
| Joan Winfield | Sally (uncredited) |
| Jack Wise | Boarder (uncredited) |
| Victor Zimmerman | Medical Officer (uncredited) |
